Timothy P. Cleland

Physical Scientist

 

Phone: 301-238-1208
Fax: 301-238-3709
E-mail Address: ClelandTP [at] si.edu

Address:
Museum Conservation Institute
Smithsonian Institution
4210 Silver Hill Road
Suitland, MD 20746


Education

Ph.D., North Carolina State University (2012)
B.S., Colorado State University (2007)

Research Specialties and Interests

  • High resolution mass spectrometry with alternative fragmentation methods
  • Sample preparation including protein extraction
  • Paleoproteomics
  • Top down (intact) proteomics
  • Bioinformatics

 

Recent Research Activities

  1. High-throughput top down proteomics with ultraviolet photodissociation

  • Development of bone protein extraction and mass spectrometry analysis
  • Paleoproteomics of bone proteins from diverse taxa

Professional Activities

2004-Present Society of Vertebrate Paleontology
2013-Present American Society for Mass Spectrometry

Recent Publications

Thomas, C. J.; Cleland, T. P.; Zhang, S.; Gundberg, C. M.; Vashishth, D., Identification and characterization of glycation adducts on osteocalcin. Analytical Biochemistry 2017, 525, 46-53.

Schroeter, E. R.; DeHart, C. J.; Cleland, T. P.; Zheng, W.; Thomas, P. M.; Kelleher, N. L.; Bern, M.; Schweitzer, M. H., Expansion for the Brachylophosaurus canadensis Collagen I Sequence and Additional Evidence of the Preservation of Cretaceous Protein. Journal of Proteome Research 2017, 16, (2), 920-932.

Cleland, T. P.; DeHart, C. J.; Fellers, R. T.; VanNispen, A. J.; Greer, J. B.; LeDuc, R. D.; Parker, W. R.; Thomas, P. M.; Kelleher, N. L.; Brodbelt, J. S., High-Throughput Analysis of Intact Human Proteins Using UVPD and HCD on an Orbitrap Mass Spectrometer. Journal of Proteome Research 2017.

Schroeter, E. R.; Cleland, T. P., Glutamine deamidation: an indicator of antiquity, or preservational quality? Rapid Communications in Mass Spectrometry 2016, 30, 251-255.

Cleland, T. P.; Thomas, C. J.; Gundberg, C. M.; Vashishth, D., Influence of carboxylation on osteocalcin detection by mass spectrometry. Rapid Communications in Mass Spectrometry 2016, 30, (19), 2109-2115.

Cleland, T. P.; Schroeter, E. R.; Feranec, R. S.; Vashishth, D., Peptide sequences from the first Castoroides ohioensis skull and the utility of old museum collections for palaeoproteomics. Proceedings of the Royal Society of London B: Biological Sciences 2016, 283, (1832).

Cleland, T. P.; Vashishth, D., Bone protein extraction without demineralization using principles from hydroxyapatite chromatography. Analytical Biochemistry 2015, 472, (0), 62-66.

Cleland, T. P.; Schroeter, E. R.; Zamdborg, L.; Zheng, W.; Lee, J. E.; Tran, J. C.; Bern, M.; Duncan, M. B.; Lebleu, V. S.; Ahlf, D. R.; Thomas, P. M.; Kalluri, R.; Kelleher, N. L.; Schweitzer, M. H., Mass Spectrometry and Antibody-Based Characterization of Blood Vessels from Brachylophosaurus canadensis. Journal of Proteome Research 2015, 14, 5252-5262.

Cleland, T. P.; Schroeter, E. R.; Schweitzer, M. H., Biologically and diagenetically derived peptide modifications in moa collagens. Proceedings of the Royal Society B: Biological Sciences 2015, 282, (1808).

Recent Presentations

Cleland, T. P., Schroeter, E. R., Feranec, R. S., Vashishth, D., Schweitzer, M. H. Beyond the Sequence: Utility of protein modidifications in fossils. Oral Presentation. Society of Vertebrate Paleontology 76th Annual Meeting Program and Abstracts. p. 118.

Schroeter, E. R., Cleland, T. P., Schweitzer, M. H. Paleoproteomics: The benefits and challenges of using Tandem Mass Spectrometry to investigate Extinct Proteomes derived from Soft-Tissues preserved in Fossil Remains. Oral Presentation Society of Vertebrate Paleontology 76th Annual Meeting Program and Abstracts p. 219.

Cleland, T.P., Schroeter, E.R., Feranec, R.S., and Vashishth, D. (2016) Protein and Post-translational modification preservation in Castoroides ohioensis from New York. Oral Presentation. Geological Society of America Abstracts with Programs 48(7).

Cleland, T.P., Feranec, R.S., and Vashishth, D. (2015) Protein preservation in Castoroides ohioensis from New York. Oral Presentation Geological Society of America Abstracts with Programs 47(3) p. 107.

Cleland, T.P., Parker, R., and Brodbelt, J.S., High-throughput Top Down Proteomics of HeLa cell lysates using UVPD. Poster Presentation. 64rd ASMS Conference on Mass Spectrometry and Allied Topics. San Antonio, TX. June 5-June 9.

Thomas, C., Cleland, T., and Vashishth, D., A proteomic analysis of fragile human bone. Poster Presentation. 64rd ASMS Conference on Mass Spectrometry and Allied Topics. San Antonio, TX. June 5-June 9.

Cleland, T., Cohen, A., Dempster, D. Recker, R. , Lappe, J., Zhou, H., Shane, E., and Vashishth, D. (2015) Advanced Glycation End-products are Higher in Trabecular Bone from Premenopausal Women with Idiopathic Osteoporosis. Poster Presentation. J Bone Miner Res 29 (Suppl 1).

Cleland, T.P., and Vashishth. D. (2015) Differential proteomics of Pteropus wing bones. Poster Presentation. 63rd ASMS Conference on Mass Spectrometry and Allied Topics. St. Louis, MO. May 31-June 4.

Cleland, T.P., Picu, C., and Vashishth, D. (2014) A New Self-healing Mechanism and its impact on Multiscale Biomechanics of Bone. Poster Presentation. First Rensselaer Workshop on Materials Stochasticity